FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182638

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182638
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16882341
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA58500.032.11969
GCCACTC79350.023.952048
CCCTTAA86050.021.252664
ACACTAT70150.020.0120898
AACACTA76300.018.1159927
AAGCCAC108150.017.9730366
AGCCACT113200.016.9804947
CACTATA100100.014.3840569
AAACACT99750.014.2901396
GTTCCCT130250.013.9382061
ACCAAAC115050.012.7168273
CAAACAC109850.012.7141075
TCCCTTA149850.012.312683
CCAAACA118700.012.1313684
CCTTAAA160750.011.6889035
CTTAAAT161800.011.4350686
CTCACTA36550.010.91206815-19
ACAAAGC186750.010.6516733
GGCCTAA38600.010.52008375-79
TACAAAG188100.010.3453292
Produced by FastQC (version 0.11.5)