FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182639

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182639
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16882341
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA61600.032.374279
GCCACTC83700.024.7702668
ACACTAT71050.022.797238
AACACTA76400.021.1031027
AAGCCAC105500.019.6486026
AGCCACT114600.018.7164387
CCCTTAA83850.018.1997014
AAACACT101800.015.9083946
CACTATA104600.015.2799619
ACCAAAC110150.014.9651983
CAAACAC114650.014.1266755
CCAAACA118200.013.8241954
GTTCCCT129100.011.9442221
TACAAAG189550.011.5490082
ACAAAGC183900.011.4296413
CTCACTA38000.010.98777715-19
CCCTAAG39250.010.932394125-129
GGCCTAA37950.010.89014175-79
TAGCCCT89100.010.7490192
TCCCTTA146700.010.5987623
Produced by FastQC (version 0.11.5)