FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182646

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182646
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13664095
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT74950.032.656954
AGTGGTA90650.027.3186365
AGCAGTG106200.023.4332832
GCAGTGG115400.021.4602783
GTGGTAT122650.020.8362546
TGGTATC154350.016.7901557
AAGCAGT150150.016.5131381
GGTATCA172100.014.7656548
GTATCAA183350.014.0559759
CACTATA50100.010.489249
AACACTA50750.010.2130477
ACACTAT57850.09.2084658
AAACACT88500.06.01931866
CAAACAC94800.05.9231775
CAACGCA97550.05.756079710-14
GCAGAGT95350.05.707893415-19
ACCAAAC104700.05.7070823
AACGCAG109050.05.043443710-14
Produced by FastQC (version 0.11.5)