FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182647

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182647
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13664095
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA109200.017.0728917
ATGGCTC107500.017.0077655
CTCAAAC113550.016.9895089
TGGCTCA110550.016.668946
AACACTA52500.013.5739367
CACTATA55450.013.2413339
GCTCAAA150100.012.9482598
TCTGATG147150.012.8228891
GATGGCT148900.012.520614
ACACTAT56550.012.3471718
CTGATGG205050.09.4085092
AAACACT92000.08.60666
TGATGGC228750.08.5281023
CTCGGGT49500.07.765461420-24
CAAACAC112800.06.8918635
ACCAAAC109250.06.78672083
CCAAACA115400.06.487064
GGGTGGG68400.05.872400325-29
GGTCCTC71550.05.51312345-49
AGGTCCT77150.05.46749545-49
Produced by FastQC (version 0.11.5)