FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182648

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182648
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13301616
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT74050.035.6786354
AGTGGTA79850.033.2673845
AGCAGTG106450.024.8674242
GTATCAA114600.024.3727479
GTGGTAT114300.023.4926
GCAGTGG116400.022.6363663
GGTATCA127400.021.1329218
TGGTATC128900.020.8311527
AAGCAGT139000.018.9844341
TCTTGGG85650.06.67414435-39
CAACGCA100350.06.140642610-14
GCAGAGT98600.06.0454815-19
TATCAAC120500.05.352767510-14
Produced by FastQC (version 0.11.5)