FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182654

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182654
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18768393
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA57600.017.248239
GCCACTC75550.013.7219548
AAGCCAC106250.09.6215246
AGCCACT110600.09.3733617
AACACTA60450.07.979337
ACACTAT60100.07.6664358
CTCACTA29400.07.3461315-19
ACAAAGC175050.06.5875323
TACAAAG165800.06.56382942
ATACAAA197350.06.30385731
CACTATA73250.06.19185549
AAACACT86500.05.4930346
CACTCAC44752.1827873E-115.475842
CCGTTAA65200.05.41102084
AAAGCCA213250.05.40151265
TACACAC43650.05.34432250-54
ATAGTGC58300.05.1864329
CGTTAAA76400.05.0884425
Produced by FastQC (version 0.11.5)