FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182658

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182658
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17116327
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACTAT66500.021.110628
CACTATA65600.020.8515229
AACACTA66700.020.5076477
AAACACT112100.012.3948066
CCAAACA118750.011.5863174
ACCAAAC126050.010.9165883
CAGTGGT76650.010.4317324
CAAACAC135300.010.2699685
GCCCTTA74950.08.1694524
AGTGGTA100700.07.7930355
TAGCCCT82950.07.64297962
AGCAGTG112200.07.64100552
GCAGTGG114150.07.005573
TATTTAG93550.07.00302469
GTGGTAT131200.06.8041816
TACCAAA229950.06.48534872
CCACTCA48850.06.4844949
ATACCAA262450.06.37356571
GTAGCCC105550.05.92572361
CTATAGC58100.05.476891510-14
Produced by FastQC (version 0.11.5)