FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182671

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182671
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17643297
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC137850.029.5024075
TGGCTCA138300.029.1988056
GGCTCAA159000.025.5336257
TCTGATG173000.024.2834381
GATGGCT165300.024.211214
CTCAAAC177000.023.3032659
GCTCAAA255450.016.400148
CCTCTAG13950.016.20200550-54
TGATGGC272450.015.3506243
CTGATGG274050.015.287052
CTCTAGG10350.015.16105650-54
TCTAGGA12250.013.2796250-54
CTAGGAT13050.012.35522850-54
CTCGGGT80150.010.29230420-24
AACACAG91700.09.81274810-14
GGGTGGG94100.08.85838125-29
AGGTCCT91800.08.84473645-49
GGTCCTC95950.08.61222645-49
ACACAGC102550.08.40951810-14
GGTGGGA108350.07.905939625-29
Produced by FastQC (version 0.11.5)