FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182676

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182676
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17258983
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA102200.049.808225
CAGTGGT105850.048.3627784
GTGGTAT137500.037.335276
GTATCAA138550.037.0003629
AGCAGTG144950.035.3666762
GGTATCA148050.035.112468
TGGTATC153500.033.5843667
AAGCAGT170800.029.6768131
GCAGTGG176350.029.3552593
GAGCACA204950.016.0897779
AGAGCAC218450.014.9306488
TCGGAAG271450.012.3868113
GATCGGA277850.012.30881
AAGAGCA283750.011.7737457
ATCGGAA302600.011.3972232
GGAAGAG354100.09.6176215
TCTTGGG119350.08.96455935-39
AGTCACA75450.08.87475125-29
GCAGAGT123050.08.77691915-19
GAAGAGC398700.08.7584656
Produced by FastQC (version 0.11.5)