FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182678

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182678
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19806188
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCAACC113600.015.6542558
AGCCCAA120950.014.4053326
GCCCAAC130350.013.9188487
CCAACCA142200.012.6072119
ATACAAG155850.011.2803211
TACAAGC164550.010.8586222
GGCCCTA33350.010.6214875-79
ACAAGCC192150.09.8613473
AAGCCCA199950.09.4699595
CCTAAGC45850.08.039943135-139
CAAGCCC235850.07.9071394
ACTCACT52800.07.036042750-54
TACGCTA54600.06.883272625-29
CTAAGCC52650.06.837447135-139
CTACTCA58600.06.41335945-49
CCCCTTA58500.06.3014197125-129
TACTCAC63850.05.931131450-54
GTACCCC70750.05.57672690-94
CTACCTA70650.05.380646745-49
CTCACTC73850.05.3229850-54
Produced by FastQC (version 0.11.5)