FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182690

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182690
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15423344
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA99500.032.924625
CAGTGGT100350.032.7174874
GTGGTAT124800.026.2499986
GTATCAA129050.025.4413039
TGGTATC131550.024.410497
GGTATCA137100.023.842458
AGCAGTG144850.022.3679682
GCAGTGG159150.020.5391123
AAGCAGT192800.017.0663911
TCTTGGG109200.06.3296735-39
Produced by FastQC (version 0.11.5)