FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15423344
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA120750.017.8881997
ATGGCTC140200.015.3552075
CTCAAAC156600.014.758629
TGGCTCA152950.014.3576346
GCTCAAA164400.013.4890528
TCTGATG168450.013.0365091
GATGGCT201450.010.9367084
CTGATGG211100.010.436762
CCTCTAG19800.08.94545650-54
TGATGGC260500.08.8721693
CTCTAGG14950.08.66889650-54
CTCGGGT71050.06.76931720-24
TCTAGGA21850.06.59038950-54
GGGTGGG76900.06.385435625-29
GGTCCTC87800.05.7567245-49
GGTGGGA92000.05.425-29
AGGTCCT100200.05.274251545-49
CAGGTCC98400.05.209756445-49
TGGGAAC101650.05.0715230-34
CTAGGAT25350.05.055621650-54
Produced by FastQC (version 0.11.5)