FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18504865
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC125750.015.4010915
GGCTCAA129050.014.8956447
TGGCTCA131900.014.1917066
CTCAAAC139800.013.6982569
TCTGATG152750.011.4641391
GATGGCT181400.010.5972154
GCTCAAA196500.09.9289788
CTCTAGG15000.09.5026550-54
CCTCTAG20000.08.92673150-54
TCTAGGA19100.07.915106350-54
CTAGGAT20000.07.84688550-54
CTCGGGT55500.07.3418120-24
CTGATGG296800.06.76782
TGATGGC341850.06.2971323
Produced by FastQC (version 0.11.5)