FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15685228
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA86600.025.187875
CAGTGGT87600.024.489444
AGCAGTG115500.018.9848162
GTGGTAT121950.018.0631666
GTATCAA137300.016.7777489
GGTATCA143050.016.0027088
TGGTATC143350.015.8185667
GCAGTGG141500.015.6702113
AAGCAGT159150.013.982291
Produced by FastQC (version 0.11.5)