FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17476305
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT23150.012.2257981
GTGAGTA31550.010.9534242
TAACCCC41600.07.9598159
TAGCATG28700.07.77531968
GTCTTAG25753.898591E-65.63658861
GTATACG94350.05.3072651
GTATAGA80250.05.24501281
GTATAGC85850.05.241011
CCTTTAG52501.6370905E-115.07401474
ATAACCC68100.05.0737918
GTATAGG73650.05.02528861
TGAGTAT70450.05.00741053
Produced by FastQC (version 0.11.5)