FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18011302
Sequences flagged as poor quality0
Sequence length150
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGAGT50000.028.3567581
GTGAGTA58300.024.232562
TGAGTAG58600.024.1085033
GAGTAGA95050.015.073894
AGAACCC104400.014.3431268
GAACCCC108450.013.6083449
TAGAACC115800.013.366297
GTAGAAC159150.09.72549256
GTTCGTT162250.08.4265081
CGTTCAG211050.07.33461764
TTCGTTC194350.07.2691452
CAGCATA200150.07.1218148
AGTAGAA227350.06.9980515
CGCCTAA47050.06.610359745-49
AGCATAT232100.06.54467879
TCGTTCA232050.06.21241143
AACCCTA53850.05.802426385-89
CTAACTA59850.05.6538763125-129
CACTCAC44051.4551915E-115.5634582
TGTGAGA45201.6370905E-105.26244163
Produced by FastQC (version 0.11.5)