FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182714

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182714
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11744209
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA67300.024.927195
CAGTGGT70750.024.5257934
AGCAGTG87700.020.1140232
GTGGTAT94250.018.3342176
TGGTATC103450.016.9821177
GTATCAA104950.016.396389
AAGCAGT113650.016.091511
GGTATCA109750.016.007298
GCAGTGG110450.015.7754643
Produced by FastQC (version 0.11.5)