FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11744209
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA91700.016.3315167
TGGCTCA87950.016.045486
CTCAAAC88800.015.8918929
ATGGCTC93000.014.9419365
TCTGATG100450.013.9054261
CCTCTAG7200.012.650-54
GCTCAAA124000.012.48387058
GATGGCT126700.011.0812954
CTCTAGG6150.09.59999950-54
CTAGGAT6650.09.09473650-54
TCTAGGA7100.08.51831150-54
CTGATGG184250.08.2062422
TGATGGC207350.07.63925743
CTCGGGT39100.07.549872420-24
CTCTAGC6450.07.144186550-54
AACACAG61850.05.77396910-14
CTAGCAC7201.627268E-85.450-54
Produced by FastQC (version 0.11.5)