FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182737

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182737
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18153987
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC138050.021.4878675
TGGCTCA142950.021.2549866
CTCAAAC156800.019.1020419
GGCTCAA161650.018.7516237
TCTGATG167750.017.4688531
GATGGCT170800.017.4098364
GCTCAAA228350.013.4320138
CTGATGG286800.010.6443522
CCTCTAG13800.010.64347850-54
TGATGGC328750.09.7241063
CTCTAGG11700.09.72307750-54
CTCGGGT71500.08.49902120-24
CTAGGAT13850.08.4216650-54
TCTAGGA14000.07.81714350-54
AACACAG111000.06.26594610-14
TCGGGTG105450.06.158748625-29
GGGTGGG108500.05.945806525-29
GGTCCTC112900.05.86713945-49
GGTGGGA121450.05.560806825-29
AGGTCCT116900.05.506244745-49
Produced by FastQC (version 0.11.5)