FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14301047
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA109100.018.5396966
CTCAAAC116900.017.9803669
GGCTCAA114350.017.8774177
ATGGCTC117000.017.4722565
TCTGATG137350.014.6517641
GATGGCT143700.014.3258774
GCTCAAA162800.012.9549538
CCTCTAG10000.011.80495550-54
CTCTAGG7500.011.32507850-54
CTGATGG215350.09.9946752
TCTAGGA9350.09.39222950-54
TGATGGC250200.08.947723
CTAGGAT9900.08.8704450-54
CTCGGGT56150.07.38416520-24
CTAGCAC6250.07.140557350-54
AACACAG76550.06.074775710-14
GGGTGGG74900.05.958596725-29
GGTCCTC77600.05.547042445-49
AGGTCCT78950.05.54336445-49
GGTGGGA86150.05.26404325-29
Produced by FastQC (version 0.11.5)