FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14883731
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA87850.031.058135
CAGTGGT89500.030.3245744
AGCAGTG126750.021.798222
GTGGTAT131350.021.3195696
GTATCAA144850.019.1834939
GCAGTGG142400.019.0096743
GGTATCA153350.017.9793458
TGGTATC157000.017.7906177
AAGCAGT165000.017.094621
TCTTGGG97250.05.981636535-39
GCAGAGT110550.05.45708615-19
CAACGCA121150.05.347849410-14