FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182746

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182746
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15759967
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT83750.046.58924
AGTGGTA85550.045.272355
GTGGTAT112650.034.891856
AGCAGTG118400.033.0168952
GGTATCA120700.032.505128
TGGTATC126300.031.1208767
GCAGTGG125450.030.9891073
GTATCAA145700.027.4712099
AAGCAGT156550.024.0770531
GCAGAGT99650.08.51028915-19
TCTTGGG101250.08.07764235-39
CAACGCA107550.08.00541510-14
CCCTAGA8450.0072866747.7203841
AACGCAG131900.06.6476110-14
CTTAGGG12450.00465509576.404371
ACGCAGA162750.05.591165515-19
TATCAAC166600.05.47043410-14
AGAGCAC118600.05.22006948
GAGCACA115400.05.1776759
Produced by FastQC (version 0.11.5)