FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182747

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182747
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15759967
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA110750.026.127776
ATGGCTC111250.025.4278585
GGCTCAA122150.023.6306937
CTCAAAC130700.022.1398479
TCTGATG139300.019.4332051
GATGGCT151900.018.812654
GCTCAAA184100.015.7570698
CTGATGG234300.012.5044382
TGATGGC247850.012.1983493
CTCGGGT63850.09.01910520-24
AACACAG78300.08.23721310-14
GGGTGGG88550.06.9098125-29
ACACAGC90950.06.759105710-14
GGTGGGA93900.06.638776325-29
GGTCCTC93900.06.53124545-49
CTCTAGG19150.06.39007950-54
TCGGGTG98950.06.299960625-29
CCTCTAG26250.06.25218250-54
TGGGAAC98600.06.191199330-34
AGGTCCT99450.06.123327745-49
Produced by FastQC (version 0.11.5)