FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13199516
Sequences flagged as poor quality0
Sequence length150
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCAGCCTCGGTGTAGATCT179670.13611862737997363Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCGTC398450.06.7576589
AGAGCGT414200.06.53546058
AAGAGCG454150.06.07111267
GAAGAGC497550.05.67176446
TAGATCT105000.05.66386140-44
GATCGGA496800.05.54870751
TCGGAAG519150.05.38633443
GGTAACT66750.05.1484761
TGTAGGG251650.05.1378236140-144
GAGTGTC180700.05.0595903140-144
ATCGGAA574400.05.00997832
Produced by FastQC (version 0.11.5)