FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182768

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182768
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16184768
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT76050.019.880414
AGTGGTA88050.017.661095
AGCAGTG110200.013.9183512
GCAGTGG120100.012.7097883
GTGGTAT127050.012.5226426
AAGCAGT155750.010.3027311
TGGTATC160050.010.3004987
GGTATCA164350.09.7681778
GTATCAA179050.09.0868329
AACACTA56700.08.6338447
ACACTAT61500.08.19418
CACTATA67850.07.6394319
CCCCTTA46600.06.7978564
AAACACT96700.05.50913766
CAAACAC101500.05.3906445
TACCCCT75150.05.0785142
Produced by FastQC (version 0.11.5)