FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182769

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182769
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16184768
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA103200.012.4176016
GGCTCAA115350.011.6089467
CTCAAAC123600.011.0668969
ATGGCTC114050.010.9206095
AACACTA56850.08.738087
GATGGCT158250.08.1892164
TCTGATG155550.07.96224551
GCTCAAA170200.07.90995658
ACACTAT61950.07.90241158
CACTATA71550.07.3452059
AAACACT95850.05.93379266
CTGATGG233050.05.8696562
CCCCTTA46351.0913936E-115.43665554
TGATGGC259700.05.23959883
CTCGGGT56650.05.159459620-24
CCTCTAG26300.05.09121750-54
CAAACAC111600.05.0318365
Produced by FastQC (version 0.11.5)