FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182773

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182773
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13833632
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA92600.013.8400197
ATGGCTC91750.013.5757745
TGGCTCA89150.013.4063736
CTCAAAC95950.013.1316959
TCTGATG94700.012.5583131
GATGGCT125650.09.8555554
GCTCAAA144250.09.4834318
CTCTAGG6100.08.49814450-54
CTAGGAT7500.07.871799550-54
CCTCTAG9650.07.4609550-54
TCTAGGA9900.05.963484350-54
CTGATGG218350.05.8050362
GATCGGA128050.05.79768941
TGATGGC270800.05.1844313
AACACAG57000.05.052590410-14
Produced by FastQC (version 0.11.5)