FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182776

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182776
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14956774
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT73350.025.7141884
AGTGGTA83000.023.15825
AGCAGTG103850.018.5440582
GCAGTGG113900.016.4970873
GTGGTAT120350.016.2697686
GGTATCA136450.014.4555838
TGGTATC137450.014.1409187
GTATCAA149400.013.395319
AAGCAGT151500.012.9202451
TCTTGGG84950.05.10202435-39
Produced by FastQC (version 0.11.5)