FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182778

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182778
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17612817
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT92400.022.204574
AGTGGTA95850.021.4799635
GTGGTAT126800.016.3505216
AGCAGTG134500.015.6542912
TGGTATC140000.015.2716367
GTATCAA156750.013.1811259
GGTATCA159400.013.1428338
GCAGTGG163300.012.9607643
AAGCAGT178550.011.7507871
CACACTA30600.010.1158917
CACTATA61800.05.70802359
ACACACT56800.05.70325236
CTAGCTA13650.05.379927625-29
ACACTAT69600.05.3787138
Produced by FastQC (version 0.11.5)