FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182785

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182785
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14121299
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCTCA110250.017.7585626
GGCTCAA118600.016.3870667
ATGGCTC120700.016.3400975
TCTGATG132150.015.2845031
CTCAAAC133550.015.0379479
GATGGCT167450.012.2079574
GCTCAAA167900.011.9183518
CTGATGG202450.010.0978832
CTCTAGG7500.09.40550950-54
TGATGGC238100.08.8278153
CTAGGAT9150.08.18143950-54
TCTAGGA9600.07.64797450-54
CCTCTAG16750.07.563369350-54
CTAGCAC7350.07.2470650-54
CTCGGGT58100.07.062087520-24
AACACAG76550.06.018333410-14
GGGTGGG70550.05.97914825-29
CCACTCA41803.6379788E-125.85505349
GGTCCTC74200.05.72351945-49
AGGTCCT73900.05.474026745-49
Produced by FastQC (version 0.11.5)