FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182797

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182797
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15723177
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA109650.024.6894687
ATGGCTC117350.023.6216435
CTCAAAC128400.021.7009339
TGGCTCA128350.021.4289056
TCTGATG165400.016.715841
GCTCAAA170450.016.4317998
GATGGCT174550.015.8395874
CCTCTAG14800.012.16216250-54
CTCTAGG12250.010.57959250-54
CTGATGG278550.010.3392572
CTCGGGT57400.09.68362420-24
TGATGGC314750.09.2644963
TCTAGGA13850.08.94151650-54
CTAGGAT15200.08.14736850-54
AGGTCCT86200.06.6988445-49
GGTCCTC88650.06.692385745-49
CTAGCAC9300.06.65806550-54
AACACAG93900.06.64025610-14
TCGGGTG96500.06.297202625-29
GGGTGGG95400.06.294339725-29
Produced by FastQC (version 0.11.5)