FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182802

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182802
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14522134
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT85150.034.5785754
AGTGGTA89200.033.6537635
AGCAGTG122550.024.4162882
GTGGTAT127100.023.4485386
GCAGTGG139850.021.4140723
GGTATCA149500.020.224728
GTATCAA152650.019.995749
TGGTATC152800.019.9757657
AAGCAGT169100.017.7109451
GAGCACA124850.07.5535519
AGAGCAC131950.06.98352968
GATCGGA134150.06.90246631
TCTTGGG96750.06.473776335-39
CAACGCA107950.06.30870910-14
GCAGAGT118950.05.567778615-19
TCGGAAG181650.05.46905333
CCTAGAC5401.0310188E-55.332778120-124
TATCAAC145050.05.141786610-14
AAGAGCA209300.05.02162847
Produced by FastQC (version 0.11.5)