FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182820

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182820
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13997196
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT69700.030.5723464
AGTGGTA76650.028.2698945
AGCAGTG104800.021.4035472
GTGGTAT106300.021.1967876
TGGTATC114800.019.2510957
GCAGTGG118850.018.535443
GGTATCA128500.017.6468168
GTATCAA126050.017.532939
AAGCAGT133250.016.9731851
GCAGAGT79450.06.23404115-19
TCTTGGG93250.05.17285435-39
Produced by FastQC (version 0.11.5)