FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182822

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182822
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17820751
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT71250.025.0573924
CCACTCA61500.023.0605669
GCCACTC73600.020.0520388
AGCAGTG120700.015.174312
AGTGGTA122050.015.1584035
GCAGTGG122050.014.8638673
AAGCCAC105400.014.2062566
AGCCACT106700.013.83077057
ACGTTAA44100.012.7328354
GTGGTAT151750.012.6659996
AAGCAGT181000.010.5132721
TACGTTA65000.010.1892813
TGGTATC220800.08.9006247
GTTACGT71800.08.8678621
CGTTAAA72600.08.8249355
ACAAAGC181250.08.81747153
GGCCTAA39700.08.12781575-79
CTCACTA36800.08.05945315-19
GTATCAA240350.07.99734749
TACAAAG205350.07.9710162
Produced by FastQC (version 0.11.5)