FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17820751
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACTCA61900.022.6764779
GCCACTC70800.019.520898
AAGCCAC104050.013.4906836
GGCTCAA108400.013.1482377
AGCCACT109400.013.0938517
ACGTTAA50100.012.7877374
CTCAAAC131800.011.4146479
TGGCTCA135750.010.0222076
TACGTTA66600.09.7281253
GTTACGT72500.09.4517991
ATGGCTC143750.09.163995
AACACTA55750.09.0382597
GATGGCT152600.09.0099064
GCTCAAA168750.08.9579198
CGTTAAA71550.08.8534895
ACAAAGC182850.08.346453
TCTGATG171500.08.3350291
ACACTAT64600.08.2457638
TACAAAG206200.07.8210552
GGCCTAA41750.07.65471775-79
Produced by FastQC (version 0.11.5)