FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16323177
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC133300.033.6504145
TGGCTCA145500.030.7298986
CTCAAAC151750.030.4606259
GGCTCAA149600.030.2727287
TCTGATG150750.029.4208951
GATGGCT183650.024.5815414
GCTCAAA216000.021.7333348
CCTCTAG16750.018.05373250-54
CTCTAGG12500.017.625650-54
CTGATGG299100.015.5747232
TCTAGGA14300.015.00419650-54
TGATGGC342600.013.9754823
CTAGGAT16100.013.86335350-54
CTCGGGT69300.012.86233820-24
CTAGCAC10800.09.650-54
AGGTCCT103800.08.98959645-49
AACACAG115050.08.94915210-14
TCGGGTG106050.08.676661525-29
GGTCCTC107700.08.62395645-49
CTCTAGC11450.08.55196550-54
Produced by FastQC (version 0.11.5)