FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182831

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182831
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15051050
Sequences flagged as poor quality0
Sequence length150
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA120800.030.0923587
ATGGCTC130950.027.704275
TGGCTCA132350.027.5204476
CTCAAAC136250.027.4726169
TCTGATG159400.022.6352041
GATGGCT172300.020.8049054
GCTCAAA184500.020.1708648
CCTCTAG15250.014.72665550-54
CTGATGG264000.013.8517472
TGATGGC282250.013.3895873
CTCGGGT55900.013.34092820-24
CTCTAGG11250.012.79666650-54
TCTAGGA15600.09.78206650-54
GAGCGTC212900.09.2642049
GATCGGA207000.09.1437121
AGAGCGT216500.09.0768488
TCGGAAG234650.08.8658253
CTAGCAC12150.08.64959850-54
TCGGGTG92850.08.46611225-29
AAGAGCG251300.07.7912687
Produced by FastQC (version 0.11.5)