FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005182954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005182954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14230673
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA113100.031.0026537
CTCAAAC120500.029.6365159
ATGGCTC124550.027.7478925
TGGCTCA130150.026.5539766
TCTGATG149950.022.6635531
GCTCAAA159950.022.1019068
GATGGCT173450.019.9250494
CTGATGG242500.014.42
TGATGGC276850.012.9514183
CCTCTAG17200.012.80930250-54
CTCGGGT63700.011.14474120-24
CTCTAGG13550.010.94612550-54
GGTTAAT53400.09.7078661
TCTAGGA18050.08.37673150-54
AACACAG94450.08.23292710-14
CTAGGAT18200.08.1494550-54
TCGGGTG95650.07.75326725-29
GGTCCTC99100.07.628658345-49
GTTAATT70600.07.54674242
AGGTCCT99950.07.534967445-49
Produced by FastQC (version 0.11.5)