FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183026

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183026
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18268661
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA111550.029.4921635
CAGTGGT116150.028.634524
AGCAGTG168500.019.6882862
GTGGTAT173000.019.3493716
GCAGTGG180300.018.3667223
GTATCAA188400.017.9607039
GGTATCA191000.017.6785188
TGGTATC196200.017.393457
AAGCAGT229200.015.0064171
CTAGCGT12100.013.44902490-94
GCTAGCG16350.010.39349285-89
CCTAAAA94350.09.0560351
TGCTAGC18850.08.93864685-89
ATGCTAG23350.07.339344585-89
AATTCTC146500.06.53612578
TTAGGGG29800.06.087894460-64
TAGGGGG34350.05.32340960-64
GCGCTAA37350.05.319874110-114
CTAAAAA175250.05.30862052
TCTTGGG129400.05.307648735-39
Produced by FastQC (version 0.11.5)