FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183095

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183095
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17585177
Sequences flagged as poor quality0
Sequence length150
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCTAACTCGTGTAGATCT374900.21319091641784443Illumina Single End PCR Primer 1 (96% over 32bp)
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC193670.11013252809454237No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA158050.040.9442527
ATGGCTC159850.040.437595
TGGCTCA171950.037.4246836
CTCAAAC179900.036.731669
TCTGATG184550.033.893311
GATGGCT220150.029.4268764
GCTCAAA225450.029.1504868
CTGATGG300000.021.427442
TGATGGC337500.019.5370663
AGAGCGT335250.018.658488
GATCGGA332150.018.6092851
CCTCTAG21250.018.56297750-54
TCGGAAG352300.017.633393
CTCTAGG15700.017.42247850-54
AAGAGCG382850.016.3951577
GAGCGTC392200.016.0594049
ATCGGAA398800.015.685732
TCTAGGA19400.014.69330450-54
CTAGGAT19200.014.3964750-54
GCTAACT87600.014.36464730-34
Produced by FastQC (version 0.11.5)