FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13659712
Sequences flagged as poor quality0
Sequence length150
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGACACACATCTCGTAT1253370.9175669296687953TruSeq Adapter, Index 4 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT214250.088.5056154
AGTGGTA226050.083.695085
AGCAGTG226050.083.6626362
GCAGTGG243250.077.986533
GTGGTAT265800.071.664296
AAGCAGT270900.069.0921551
TGGTATC288350.066.009967
GGTATCA297700.064.396218
GTATCAA300100.063.761279
AGAGCAC357050.057.3615958
GAGCACA359600.056.9548349
GATCGGA406400.051.1195831
TCGGAAG460800.044.933293
AAGAGCA464600.044.4858557
ATCGGAA494100.042.129362
GAAGAGC505850.040.900916
GGAAGAG534350.039.016825
CGGAAGA569600.036.3871154
CGACACA234150.018.14049130-34
TCTCGTA229750.018.1246140-44
Produced by FastQC (version 0.11.5)