FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14615328
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT81750.031.3541284
AGTGGTA98550.025.7899555
AGCAGTG113550.022.763542
GTGGTAT120750.021.2869556
GCAGTGG124100.020.7703483
GGTATCA126250.020.5306918
TGGTATC126950.020.3607717
GTATCAA152250.017.1665029
AAGCAGT170100.015.7460321
TTAGTCC46150.011.7009749
ACCCACT57950.09.9396042
TTTAGTC67300.08.9866278
CACTTTA76900.07.67750365
CCCACTT86150.07.52176433
GAGCACA140600.07.42531979
CACCCAC94900.06.82824041
AGAGCAC154650.06.79728368
GATCGGA161700.06.5899811
CCACTTT105000.06.30857134
CAACGCA96650.06.138437310-14
Produced by FastQC (version 0.11.5)