FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14615328
Sequences flagged as poor quality0
Sequence length150
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCAA99000.018.5454547
TGGCTCA108900.017.4545466
ATGGCTC109350.016.724285
CTCAAAC122900.015.290489
TTAGTCC43450.014.08515559
GATGGCT142400.013.247194
TCTGATG151000.012.11125851
GCTCAAA166450.011.4196448
ACCCACT60050.010.7910082
TTTAGTC67200.010.2857158
CTGATGG223550.09.1791542
TGATGGC229600.08.8118473
CACTTTA76600.08.6475195
CTCGGGT46100.07.99652920-24
CCCACTT88200.07.34693863
CCACTTT102400.06.82031254
GATCGGA165200.06.4503631
CACCCAC105650.06.40605741
TCGGAAG169400.06.2054313
AGAGCGT192400.05.91268168
Produced by FastQC (version 0.11.5)