FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15036504
Sequences flagged as poor quality0
Sequence length150
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA86650.039.80155
CAGTGGT88800.039.2432444
GTGGTAT116450.030.2344366
AGCAGTG117250.029.9053292
GTATCAA127850.027.989059
TGGTATC131950.026.6828357
GGTATCA136950.026.2343928
AAGCAGT143550.024.92791
GCAGTGG139800.024.9270363
GCAGAGT103500.07.24869615-19
TCTTGGG108650.06.746065635-39
TATCAAC129700.06.4172710-14
CAACGCA126350.06.40506510-14
AACGCAG139550.05.757936510-14
Produced by FastQC (version 0.11.5)