FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14810711
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGGT85800.032.889944
AGTGGTA89350.031.7443165
AGCAGTG116550.024.503572
GTGGTAT125300.023.1531186
GCAGTGG133100.021.580823
TGGTATC146050.019.9129377
GTATCAA158950.018.4327189
AAGCAGT158550.018.3790281
GGTATCA159250.018.3075878
CACTATA49000.06.6110739
GCAGAGT102250.06.25197915-19
TACACTA34601.0677468E-95.82555688
AACACTA49750.05.78791867
ACACTAT52500.05.75897938
TCTTGGG109450.05.446552835-39
CAACGCA126050.05.345516710-14
AACGCAG130250.05.01839410-14
Produced by FastQC (version 0.11.5)