FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15020512
Sequences flagged as poor quality0
Sequence length150
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGGTA67700.023.0745165
CAGTGGT75250.020.281684
GTGGTAT95050.016.1320326
GTATCAA104850.014.8992519
AGCAGTG109500.014.2884232
TGGTATC120050.013.3121447
GGTATCA122000.012.9229968
AAGCAGT137850.011.8069361
GCAGTGG137900.011.0674143
Produced by FastQC (version 0.11.5)