FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13509399
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATAGACAATCTCGTAT150470.11138171283563393TruSeq Adapter, Index 9 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGAGTG50700.020.3763921
GAGCACA132350.019.4742559
AGAGCAC134950.019.0994828
TGAGTGA55000.018.9955222
GAGTGAT56400.018.9072573
GATCGGA151300.017.0939851
TCGGAAG174650.014.7693143
AGTGATG82750.013.8344864
ATCGGAA189850.013.6247872
AAGAGCA208650.012.4911267
ATGGTTG99050.010.6849958
GGAAGAG259050.010.3944015
GATGGTT109400.010.2664127
GAAGAGC267250.09.96771056
CGGAAGA284050.09.6574864
TGGTTGA114950.09.582629
TAGTGTG25350.08.69054715-19
AGTCACG71850.07.535283625-29
CAGTCAC74850.07.32945525-29
CCAGTCA80200.06.948244625-29
Produced by FastQC (version 0.11.5)