FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13509399
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTGCACTGTCGTGTAGATCT159270.11789569617419694Illumina Single End PCR Primer 1 (96% over 32bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA154700.016.4342271
GTGAGTG48800.015.3489411
TCGGAAG179950.014.5244293
TGAGTGA51600.014.0947092
GAGTGAT52950.013.7340833
AGAGCGT193750.013.4886168
ATCGGAA196850.013.16896252
AAGAGCG229300.011.2089957
GAGCGTC232550.011.0523019
GGAAGAG270500.09.8479675
GAAGAGC272300.09.6506686
AGTGATG82950.09.4606784
CGGAAGA294800.08.8896784
TAGATCT60700.08.65831840-44
ATGGTTG96950.08.4656348
GTGCACT61700.08.42478830-34
TAGTGTG23450.07.55258715-19
TGGTTGA114450.07.4227899
GATGGTT111950.07.2670287
AGTGTGC76300.06.86929125-29
Produced by FastQC (version 0.11.5)