FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13023467
Sequences flagged as poor quality0
Sequence length150
%GC57

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG77000.014.1349491
TGGCTCA87750.012.4716516
CTCAAAC90500.012.411059
ATGGCTC87600.012.4108165
CCTCTAG5350.011.57346750-54
GGCTCAA95000.011.51999957
CTCTAGG4000.09.35970550-54
GATGGCT119650.09.2667084
GCTCAAA140750.08.3893438
TCTAGGA4653.6379788E-128.05135950-54
CTAGGAT5653.6925485E-106.6263450-54
CTGATGG204100.05.82177162
TGATGGC242200.05.32107933
CTAGCAC4802.8526835E-45.09983950-54
Produced by FastQC (version 0.11.5)