FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005183195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005183195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16818869
Sequences flagged as poor quality0
Sequence length150
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGCTC151750.029.7489285
GGCTCAA152250.029.0364537
CTCAAAC164900.028.1625219
TCTGATG184050.023.6283631
GCTCAAA196800.023.2682918
GATGGCT199250.022.6569634
TGGCTCA212550.021.3747356
CTGATGG296200.015.3139772
CCTCTAG21350.014.97330350-54
TGATGGC345300.013.8662033
CTCTAGG16750.012.20776150-54
TCTAGGA19050.010.80944850-54
CTCGGGT87000.010.54344820-24
CTAGGAT21250.09.62258850-54
AACACAG114900.09.04856410-14
CTAGCAC16100.08.40745450-54
TGGGAAC116800.08.14931530-34
GGTCCTC127000.08.12976445-49
AGGTCCT123500.08.10364345-49
TCGGGTG120750.07.691925525-29
Produced by FastQC (version 0.11.5)